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KMID : 0545120170270030610
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 3 p.610 ~ p.615
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Host Cell Nuclear Localization of Shigella flexneri Effector OspF Is Facilitated by SUMOylation
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Jo Kyung-Min
Kim Eun-Jin
Yu Hyun-Jin
Yun Cheol-Heui
Kim Dong-Wook
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Abstract
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When Shigella infect host cells, various effecter molecules are delivered into the cytoplasm of the host cell through the type III secretion system (TTSS) to facilitate their invasion process and control the host immune responses. Among these effectors, the S. flexneri effector OspF dephosphorylates mitogen-activated protein kinases and translocates itself to the nucleus, thus preventing histone H3 modification to regulate expression of proinflammatory cytokines. Despite the critical role of OspF, the mechanism by which it localizes in the nucleus has remained to be elucidated. In the present study, we identified a potential small ubiquitinrelated modifier (SUMO) modification site within OspF and we demonstrated that Shigella TTSS effector OspF is conjugated with SUMO in the host cell and this modification mediates the nuclear translocation of OspF. Our results show a bacterial virulence factor can exploit host post-translational machinery to execute its intracellular trafficking.
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KEYWORD
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Shigella flexneri, effector, OspF, SUMO, nucelar localization
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